+1 |BT| (S1 (S (NP (NP (NN Suppression)) (PP (IN of) (NP (NP (NN rat) (NN thromboxane) (NN synthase) (NN gene)) (NN transcription))) (PP (IN by) (NP (NP (NN _PROT1_) (JJ _PROT1_) (NN _PROT1_) (NN _PROT1_)) (PP (IN in) (NP (NNS macrophages))))) (PP (IN via) (NP (NP (DT an) (NN interaction)) (PP (IN with) (NP (NN _PROT2_)))))))) |ET|
-1 |BT| (S1 (S (NP (NN Deletion) (NN analysis)) (VP (VBD showed) (SBAR (IN that) (S (NP (NP (DT the) (NN element)) (ADJP (JJ responsible) (PP (IN for) (NP (DT the) (NN PPARgamma) (NN effect))))) (VP (VBZ is) (ADJP (JJ located) (PP (IN in) (NP (NP (DT a) (NN region)) (VP (VBG containing) (NP (NP (DT the) (JJ nuclear) (NN factor) (NN E2) (-LRB- -LRB-) (NN _PROT1_) (-RRB- -RRB-) (NN _PROT2_) (NN site)) (PRN (-LRB- -LRB-) (NP (NN -98/-88)) (-RRB- -RRB-))))))) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN indicated) (S (VP (TO to) (VP (VB be) (NP (NP (DT the) (JJ major) (NN promoter)) (PP (IN of) (NP (DT the) (NN TXS) (NN gene))))))))))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NP (JJ electrophoretic) (NN mobility) (NN shift) (NN assay)) (VP (VBG using) (NP (NP (DT the) (NN _PROT1_AND_PROT2_) (NN site)) (CC and) (NP (NP (JJ nuclear) (NNS extracts)) (PP (IN from) (NP (NNS macrophages)))))))) (, ,) (NP (PRP we)) (VP (VBD observed) (NP (NP (DT a) (JJ specific) (JJ protein-DNA) (NN complex) (NN formation)) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN inhibited) (PP (IN by) (NP (NP (DT a) (JJ specific) (NN antibody)) (PP (IN against) (NP (DT the) (NN transcription) (NN factor) (NP (NP (NN _PROT_)) (PRN (-LRB- -LRB-) (NP (JJ _PROT_) (NN _PROT_) (CD _PROT_)) (-RRB- -RRB-))))))))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NP (JJ electrophoretic) (NN mobility) (NN shift) (NN assay)) (VP (VBG using) (NP (NP (DT the) (NN _PROT1_) (NN site)) (CC and) (NP (NP (JJ nuclear) (NNS extracts)) (PP (IN from) (NP (NNS macrophages)))))))) (, ,) (NP (PRP we)) (VP (VBD observed) (NP (NP (DT a) (JJ specific) (JJ protein-DNA) (NN complex) (NN formation)) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN inhibited) (PP (IN by) (NP (NP (DT a) (JJ specific) (NN antibody)) (PP (IN against) (NP (DT the) (NN transcription) (NN factor) (NP (NP (NN _PROT2_)) (PRN (-LRB- -LRB-) (NP (JJ _PROT_) (NN _PROT_) (CD _PROT_)) (-RRB- -RRB-))))))))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NP (JJ electrophoretic) (NN mobility) (NN shift) (NN assay)) (VP (VBG using) (NP (NP (DT the) (NN _PROT1_) (NN site)) (CC and) (NP (NP (JJ nuclear) (NNS extracts)) (PP (IN from) (NP (NNS macrophages)))))))) (, ,) (NP (PRP we)) (VP (VBD observed) (NP (NP (DT a) (JJ specific) (JJ protein-DNA) (NN complex) (NN formation)) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN inhibited) (PP (IN by) (NP (NP (DT a) (JJ specific) (NN antibody)) (PP (IN against) (NP (DT the) (NN transcription) (NN factor) (NP (NP (NN _PROT_)) (PRN (-LRB- -LRB-) (NP (JJ _PROT2_) (NN _PROT2_) (CD _PROT2_)) (-RRB- -RRB-))))))))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NP (JJ electrophoretic) (NN mobility) (NN shift) (NN assay)) (VP (VBG using) (NP (NP (DT the) (NN _PROT1_) (NN site)) (CC and) (NP (NP (JJ nuclear) (NNS extracts)) (PP (IN from) (NP (NNS macrophages)))))))) (, ,) (NP (PRP we)) (VP (VBD observed) (NP (NP (DT a) (JJ specific) (JJ protein-DNA) (NN complex) (NN formation)) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN inhibited) (PP (IN by) (NP (NP (DT a) (JJ specific) (NN antibody)) (PP (IN against) (NP (DT the) (NN transcription) (NN factor) (NP (NP (NN _PROT2_)) (PRN (-LRB- -LRB-) (NP (JJ _PROT_) (NN _PROT_) (CD _PROT_)) (-RRB- -RRB-))))))))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NP (JJ electrophoretic) (NN mobility) (NN shift) (NN assay)) (VP (VBG using) (NP (NP (DT the) (NN _PROT1_) (NN site)) (CC and) (NP (NP (JJ nuclear) (NNS extracts)) (PP (IN from) (NP (NNS macrophages)))))))) (, ,) (NP (PRP we)) (VP (VBD observed) (NP (NP (DT a) (JJ specific) (JJ protein-DNA) (NN complex) (NN formation)) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN inhibited) (PP (IN by) (NP (NP (DT a) (JJ specific) (NN antibody)) (PP (IN against) (NP (DT the) (NN transcription) (NN factor) (NP (NP (NN _PROT_)) (PRN (-LRB- -LRB-) (NP (JJ _PROT2_) (NN _PROT2_) (CD _PROT2_)) (-RRB- -RRB-))))))))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NP (JJ electrophoretic) (NN mobility) (NN shift) (NN assay)) (VP (VBG using) (NP (NP (DT the) (NN _PROT_) (NN site)) (CC and) (NP (NP (JJ nuclear) (NNS extracts)) (PP (IN from) (NP (NNS macrophages)))))))) (, ,) (NP (PRP we)) (VP (VBD observed) (NP (NP (DT a) (JJ specific) (JJ protein-DNA) (NN complex) (NN formation)) (, ,) (SBAR (WHNP (WDT which)) (S (VP (VBD was) (VP (VBN inhibited) (PP (IN by) (NP (NP (DT a) (JJ specific) (NN antibody)) (PP (IN against) (NP (DT the) (NN transcription) (NN factor) (NP (NP (NN _PROT1_)) (PRN (-LRB- -LRB-) (NP (JJ _PROT2_) (NN _PROT2_) (CD _PROT2_)) (-RRB- -RRB-))))))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Finally)) (, ,) (NP (NP (DT a) (JJ direct) (NN interaction)) (PP (IN between) (NP (NP (NN PPARgamma)) (CC and) (NP (NN _PROT1_))))) (VP (VBD was) (VP (VBN confirmed) (PP (IN by) (NP (NP (NN _PROT2_) (NN _PROT2_)) (JJ pull-down) (NN assay))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (, ,) (NP (PRP we)) (VP (VP (VBD cloned)) (CC and) (VP (VBD characterized) (NP (NP (DT a) (NN mouse) (NN orthologue)) (PP (IN of) (NP (JJ human) (NN _PROT1_) (NN protein)))) (PP (IN as) (NP (DT an) (JJ atypical) (NN _PROT2_))) (PRN (-LRB- -LRB-) (NP (VBN named) (NN _PROT_) (NN _PROT_)) (-RRB- -RRB-)))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (, ,) (NP (PRP we)) (VP (VP (VBD cloned)) (CC and) (VP (VBD characterized) (NP (NP (DT a) (NN mouse) (NN orthologue)) (PP (IN of) (NP (JJ human) (NN _PROT1_) (NN protein)))) (PP (IN as) (NP (DT an) (JJ atypical) (NN _PROT_))) (PRN (-LRB- -LRB-) (NP (VBN named) (NN _PROT2_) (NN _PROT2_)) (-RRB- -RRB-)))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (, ,) (NP (PRP we)) (VP (VP (VBD cloned)) (CC and) (VP (VBD characterized) (NP (NP (DT a) (NN mouse) (NN orthologue)) (PP (IN of) (NP (JJ human) (NN _PROT_) (NN protein)))) (PP (IN as) (NP (DT an) (JJ atypical) (NN _PROT1_))) (PRN (-LRB- -LRB-) (NP (VBN named) (NN _PROT2_) (NN _PROT2_)) (-RRB- -RRB-)))))) |ET|
-1 |BT| (S1 (S (NP (NP (JJ Subcellular) (NN fractionation)) (CC and) (NP (JJ antibody-uptake) (NNS experiments))) (VP (VBP indicate) (SBAR (IN that) (S (NP (NN _PROT1_) (CD _PROT1_)) (VP (VP (VBZ is) (ADVP (RB indeed)) (NP (DT a) (JJ type-I) (NN membrane) (NN protein))) (, ,) (CC but) (, ,) (PP (IN unlike) (NP (JJ other) (NN _PROT2_) (NNS isoforms))) (, ,) (VP (VBZ lacks) (NP (DT an) (JJ N-terminal) (JJ extracellular) (NN domain))))))))) |ET|
+1 |BT| (S1 (S (NP (NP (JJ Direct) (NN interaction)) (PP (IN between) (NP (NP (NP (DT the) (JJ cytoplasmic) (NN tail)) (PP (IN of) (NP (NN _PROT1_) (CD _PROT1_)))) (CC and) (NP (NP (DT the) (NN Src) (NN homology) (CD 3) (NN domain)) (PP (IN of) (NP (NN _PROT2_))))))) (VP (VBZ activates) (NP (NP (NN _PROT_) (NN _PROT_)) (PP (IN in) (NP (NN C2C12) (NNS cells))))))) |ET|
+1 |BT| (S1 (S (NP (NP (JJ Direct) (NN interaction)) (PP (IN between) (NP (NP (NP (DT the) (JJ cytoplasmic) (NN tail)) (PP (IN of) (NP (NN _PROT1_) (CD _PROT1_)))) (CC and) (NP (NP (DT the) (NN Src) (NN homology) (CD 3) (NN domain)) (PP (IN of) (NP (NN _PROT_))))))) (VP (VBZ activates) (NP (NP (NN _PROT2_) (NN _PROT2_)) (PP (IN in) (NP (NN C2C12) (NNS cells))))))) |ET|
+1 |BT| (S1 (S (NP (NP (JJ Direct) (NN interaction)) (PP (IN between) (NP (NP (NP (DT the) (JJ cytoplasmic) (NN tail)) (PP (IN of) (NP (NN _PROT_) (CD _PROT_)))) (CC and) (NP (NP (DT the) (NN Src) (NN homology) (CD 3) (NN domain)) (PP (IN of) (NP (NN _PROT1_))))))) (VP (VBZ activates) (NP (NP (NN _PROT2_) (NN _PROT2_)) (PP (IN in) (NP (NN C2C12) (NNS cells))))))) |ET|
+1 |BT| (S1 (S (NP (DT This)) (VP (VBZ suggests) (SBAR (IN that) (S (NP (NN transmembrane) (NN _PROT1_) (CD _PROT1_)) (, ,) (PP (IN by) (S (VP (VBG providing) (NP (NP (NN docking) (NNS sites)) (PP (IN for) (NP (NP (DT the) (NN Src) (NN homology) (CD 3) (NN domain)) (PP (IN of) (NP (NN _PROT2_))))))))) (, ,) (VP (VBZ activates) (NP (NN PI) (NN 3-kinase)) (PP (IN by) (S (VP (VBG mediating) (NP (NP (PRP$ its) (NN recruitment)) (PP (TO to) (NP (DT the) (NN membrane))))))))))))) |ET|
+1 |BT| (S1 (S (NP (NP (NN Modification)) (PP (IN of) (NP (ADJP (FW de) (FW novo)) (NN DNA) (NN methyltransferase) (NN 3a) (-LRB- -LRB-) (NN _PROT1_) (-RRB- -RRB-))) (PP (IN by) (NP (NN _PROT2_)))) (VP (VBZ modulates) (NP (NP (NP (PRP$ its) (NN interaction)) (PP (IN with) (NP (NP (NN histone) (NNS deacetylases)) (PRN (-LRB- -LRB-) (NP (NNS HDACs)) (-RRB- -RRB-))))) (CC and) (NP (PRP$ its) (NN capacity) (S (VP (TO to) (VP (VB repress) (NP (NN transcription)))))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT1_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT2_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT_)) (CC and) (NP (NN _PROT_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT1_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT2_)) (CC and) (NP (NN _PROT_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT1_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT_)) (CC and) (NP (NN _PROT2_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT1_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT_)) (CC and) (NP (NN _PROT_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT2_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT1_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT2_)) (CC and) (NP (NN _PROT_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT1_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT_)) (CC and) (NP (NN _PROT2_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT1_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT_)) (CC and) (NP (NN _PROT_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT2_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT1_)) (CC and) (NP (NN _PROT2_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT1_)) (CC and) (NP (NN _PROT_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT2_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Here)) (NP (PRP we)) (VP (VBP show) (SBAR (IN that) (S (NP (NN _PROT_)) (VP (VBZ interacts) (PP (IN with) (NP (NP (NP (JJ multiple) (NNS components)) (PP (IN of) (NP (DT the) (NN sumoylation) (NN machinery)))) (, ,) (RB namely) (NP (NP (DT the) (NN E2) (NN sumo) (NN conjugating) (NN enzyme) (NN _PROT_)) (CC and) (NP (NP (DT the) (NN E3) (NN sumo) (NNS ligases)) (NP (NP (NN _PROT_)) (CC and) (NP (NN _PROT1_))))) (, ,) (SBAR (WHNP (NP (DT all)) (WHPP (IN of) (WHNP (WDT which)))) (S (VP (VBP are) (VP (VBN involved) (PP (IN in) (S (VP (VBG conjugating) (NP (NP (DT the) (NP (JJ small) (JJ ubiquitin-like) (NN modifier)) (NN polypeptide)) (, ,) (NP (NN _PROT2_)) (, ,)) (PP (TO to) (NP (PRP$ its) (NN target) (NNS proteins)))))))))))))))))) |ET|
+1 |BT| (S1 (S (S (NP (NN _PROT1_)) (VP (VBZ is) (VP (VBN modified) (PP (IN by) (NP (NN _PROT2_))) (ADVP (ADVP (FW in) (FW vivo)) (CC and) (ADVP (FW in) (FW vitro)))))) (CC and) (S (NP (NP (DT the) (NN region)) (PP (IN of) (NP (NN _PROT_))) (ADJP (JJ responsible) (PP (IN for) (NP (NN interaction))))) (VP (VBZ maps) (PP (TO to) (NP (DT the) (JJ N-terminal) (JJ regulatory) (NN domain))))))) |ET|
-1 |BT| (S1 (S (S (NP (NN _PROT1_)) (VP (VBZ is) (VP (VBN modified) (PP (IN by) (NP (NN _PROT_))) (ADVP (ADVP (FW in) (FW vivo)) (CC and) (ADVP (FW in) (FW vitro)))))) (CC and) (S (NP (NP (DT the) (NN region)) (PP (IN of) (NP (NN _PROT2_))) (ADJP (JJ responsible) (PP (IN for) (NP (NN interaction))))) (VP (VBZ maps) (PP (TO to) (NP (DT the) (JJ N-terminal) (JJ regulatory) (NN domain))))))) |ET|
-1 |BT| (S1 (S (S (NP (NN _PROT_)) (VP (VBZ is) (VP (VBN modified) (PP (IN by) (NP (NN _PROT1_))) (ADVP (ADVP (FW in) (FW vivo)) (CC and) (ADVP (FW in) (FW vitro)))))) (CC and) (S (NP (NP (DT the) (NN region)) (PP (IN of) (NP (NN _PROT2_))) (ADJP (JJ responsible) (PP (IN for) (NP (NN interaction))))) (VP (VBZ maps) (PP (TO to) (NP (DT the) (JJ N-terminal) (JJ regulatory) (NN domain))))))) |ET|
+1 |BT| (S1 (S (ADVP (RB Functionally)) (, ,) (NP (NP (NN sumoylation)) (PP (IN of) (NP (NN _PROT1_)))) (VP (VBZ disrupts) (NP (PRP$ its) (NN ability) (S (VP (TO to) (VP (VB interact) (PP (PP (IN with) (NP (NP (NN histone) (NNS deacetylases)) (PRN (-LRB- -LRB-) (NP (NN _PROT2_)) (-RRB- -RRB-)))) (, ,) (CC but) (PP (RB not) (IN with) (NP (NP (DT another) (NN interaction) (NN partner)) (, ,) (NP (NN _PROT_)))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Functionally)) (, ,) (NP (NP (NN sumoylation)) (PP (IN of) (NP (NN _PROT1_)))) (VP (VBZ disrupts) (NP (PRP$ its) (NN ability) (S (VP (TO to) (VP (VB interact) (PP (PP (IN with) (NP (NP (NN histone) (NNS deacetylases)) (PRN (-LRB- -LRB-) (NP (NN _PROT_)) (-RRB- -RRB-)))) (, ,) (CC but) (PP (RB not) (IN with) (NP (NP (DT another) (NN interaction) (NN partner)) (, ,) (NP (NN _PROT2_)))))))))))) |ET|
-1 |BT| (S1 (S (ADVP (RB Functionally)) (, ,) (NP (NP (NN sumoylation)) (PP (IN of) (NP (NN _PROT_)))) (VP (VBZ disrupts) (NP (PRP$ its) (NN ability) (S (VP (TO to) (VP (VB interact) (PP (PP (IN with) (NP (NP (NN histone) (NNS deacetylases)) (PRN (-LRB- -LRB-) (NP (NN _PROT1_)) (-RRB- -RRB-)))) (, ,) (CC but) (PP (RB not) (IN with) (NP (NP (DT another) (NN interaction) (NN partner)) (, ,) (NP (NN _PROT2_)))))))))))) |ET|
+1 |BT| (S1 (S (NP (NN _PROT1_)) (VP (VBZ associates) (PP (IN with) (NP (NP (NN _PROT2_) (NN _PROT2_) (NN _PROT2_) (CD _PROT2_)) (, ,) (NP (NN Syk)) (, ,) (CC and) (NP (NN phospholipase) (NN C-gamma))))) (LST (-LRB- -LRB-) (LS 1) (-RRB- -RRB-)) (PP (IN upon) (NP (NN B) (NN cell) (NN activation))))) |ET|
+1 |BT| (S1 (S (NP (DT These) (NNS results)) (VP (VBP suggest) (SBAR (IN that) (S (NP (VBN tyrosyl-phosphorylated) (NN _PROT1_)) (VP (MD may) (VP (VB be) (NP (DT a) (NN substrate)) (SBAR (IN for) (S (NP (NN _PROT2_)) (VP (VBZ regulates) (NP (NP (NN tyrosyl) (NN phosphorylation)) (PP (IN of) (NP (NN _PROT_)))))))))))))) |ET|
-1 |BT| (S1 (S (NP (DT These) (NNS results)) (VP (VBP suggest) (SBAR (IN that) (S (NP (VBN tyrosyl-phosphorylated) (NN _PROT1_)) (VP (MD may) (VP (VB be) (NP (DT a) (NN substrate)) (SBAR (IN for) (S (NP (NN _PROT_)) (VP (VBZ regulates) (NP (NP (NN tyrosyl) (NN phosphorylation)) (PP (IN of) (NP (NN _PROT2_)))))))))))))) |ET|
+1 |BT| (S1 (S (NP (DT These) (NNS results)) (VP (VBP suggest) (SBAR (IN that) (S (NP (VBN tyrosyl-phosphorylated) (NN _PROT_)) (VP (MD may) (VP (VB be) (NP (DT a) (NN substrate)) (SBAR (IN for) (S (NP (NN _PROT1_)) (VP (VBZ regulates) (NP (NP (NN tyrosyl) (NN phosphorylation)) (PP (IN of) (NP (NN _PROT2_)))))))))))))) |ET|
+1 |BT| (S1 (S (NP (NP (DT The) (NN association)) (PP (IN of) (NP (NN _PROT1_))) (PP (IN with) (NP (NN _PROT2_)))) (VP (VBD was) (VP (VBN mediated) (PP (IN by) (NP (NP (DT the) (JJ NH2-terminal) (NN Src) (NN homology) (CD 2) (-LRB- -LRB-) (NN SH2) (-RRB- -RRB-) (NN domain)) (PP (IN of) (NP (NN _PROT_))))))))) |ET|
-1 |BT| (S1 (S (NP (NP (DT The) (NN association)) (PP (IN of) (NP (NN _PROT1_))) (PP (IN with) (NP (NN _PROT_)))) (VP (VBD was) (VP (VBN mediated) (PP (IN by) (NP (NP (DT the) (JJ NH2-terminal) (NN Src) (NN homology) (CD 2) (-LRB- -LRB-) (NN SH2) (-RRB- -RRB-) (NN domain)) (PP (IN of) (NP (NN _PROT2_))))))))) |ET|
+1 |BT| (S1 (S (NP (NP (DT The) (NN association)) (PP (IN of) (NP (NN _PROT_))) (PP (IN with) (NP (NN _PROT1_)))) (VP (VBD was) (VP (VBN mediated) (PP (IN by) (NP (NP (DT the) (JJ NH2-terminal) (NN Src) (NN homology) (CD 2) (-LRB- -LRB-) (NN SH2) (-RRB- -RRB-) (NN domain)) (PP (IN of) (NP (NN _PROT2_))))))))) |ET|
+1 |BT| (S1 (S (NP (NP (NNS Complexes)) (PP (IN of) (NP (DT either) (NN _PROT1_AND_PROT2_)))) (LST (-LRB- -LRB-) (LS 1) (-RRB- -RRB-)) (VP (MD could) (VP (VB be) (VP (VBN isolated) (PP (IN from) (NP (NP (NN B) (NNS cells)) (VP (VBN stimulated) (PP (IN by) (NP (NP (NP (NN BCR)) (NN engagement)) (CC or) (NP (NP (DT a) (NN mixture)) (PP (IN of) (NP (NP (NN hydrogen) (NN peroxidase)) (CC and) (NP (NN sodium) (NN orthovanadate)))))))))) (, ,) (ADVP (RB respectively))))))) |ET|
-1 |BT| (S1 (S (NP (NP (JJ Transient) (NN expression)) (PP (IN of) (NP (NP (NN _PROT1_)) (CC and) (NP (NP (NP (DT a) (JJ null) (NN mutant)) (PP (IN of) (NP (NN _PROT2_)))) (PRN (-LRB- -LRB-) (NP (NN PTP-1CM)) (-RRB- -RRB-))))) (PP (IN in) (NP (NN COS) (NNS cells)))) (VP (VBD resulted) (PP (IN in) (NP (NP (NP (DT an) (NN increase)) (PP (IN in) (NP (NP (NN tyrosyl) (NN phosphorylation)) (PP (IN of) (NP (NN _PROT_)))))) (CC and) (NP (NP (PRP$ its) (NN interaction)) (PP (IN with) (NP (NN PTP-1CM))))))))) |ET|
-1 |BT| (S1 (S (NP (NP (JJ Transient) (NN expression)) (PP (IN of) (NP (NP (NN _PROT1_)) (CC and) (NP (NP (NP (DT a) (JJ null) (NN mutant)) (PP (IN of) (NP (NN _PROT_)))) (PRN (-LRB- -LRB-) (NP (NN PTP-1CM)) (-RRB- -RRB-))))) (PP (IN in) (NP (NN COS) (NNS cells)))) (VP (VBD resulted) (PP (IN in) (NP (NP (NP (DT an) (NN increase)) (PP (IN in) (NP (NP (NN tyrosyl) (NN phosphorylation)) (PP (IN of) (NP (NN _PROT2_)))))) (CC and) (NP (NP (PRP$ its) (NN interaction)) (PP (IN with) (NP (NN PTP-1CM))))))))) |ET|
+1 |BT| (S1 (S (NP (NP (JJ Transient) (NN expression)) (PP (IN of) (NP (NP (NN _PROT_)) (CC and) (NP (NP (NP (DT a) (JJ null) (NN mutant)) (PP (IN of) (NP (NN _PROT1_)))) (PRN (-LRB- -LRB-) (NP (NN PTP-1CM)) (-RRB- -RRB-))))) (PP (IN in) (NP (NN COS) (NNS cells)))) (VP (VBD resulted) (PP (IN in) (NP (NP (NP (DT an) (NN increase)) (PP (IN in) (NP (NP (NN tyrosyl) (NN phosphorylation)) (PP (IN of) (NP (NN _PROT2_)))))) (CC and) (NP (NP (PRP$ its) (NN interaction)) (PP (IN with) (NP (NN PTP-1CM))))))))) |ET|
-1 |BT| (S1 (S (PP (IN By) (NP (NN contrast))) (, ,) (NP (NN _PROT1_)) (VP (VP (VBD was) (RB not) (ADJP (NN tyrosyl) (JJ phosphorylated))) (CC or) (VP (VBD associated) (PP (IN with) (NP (NN PTP-1CM))) (PP (IN in) (DT the) (NN presence) (IN of) (NP (JJ wild-type) (NN _PROT2_))))))) |ET|
